| 2025-05-14 19:30:31May 14, 2025 | Ambassadors Ohio U23 @ Croatia Cleveland | 1 - 3 | Mentor High School |
| 2025-05-17 17:00:07May 17, 2025 | Ambassadors FC Ohio @ Syracuse FC | 4 - 0 | Camden High School |
| 2025-05-24 19:00:54May 24, 2025 | Ambassadors FC Ohio vs Flower City Union | 1 - 2 | Malone University Kingfield |
| 2025-05-28 19:00:40May 28, 2025 | Ambassadors FC Ohio vs Cleveland SC | 1 - 1 | Malone University Kingfield |
| 2025-05-31 19:00:08May 31, 2025 | Ambassadors FC Ohio @ Niagara 1812 | 19:00:087:00 pm | Niagara University |
| 2025-06-01 13:00:33June 1, 2025 | Ambassadors Ohio U23 vs FC Pittsburgh | 13:00:331:00 pm | Malone University Kingfield |
| 2025-06-04 19:00:35June 4, 2025 | Ambassadors FC Ohio @ Cleveland SC | 19:00:357:00 pm | Finnie Stadium |
| 2025-06-07 13:00:01June 7, 2025 | Ambassadors Ohio U23 vs Ohio Premier | 13:00:011:00 pm | Malone University Kingfield |
| 2025-06-11 19:00:20June 11, 2025 | Ambassadors Ohio U23 vs WV Highlanders | 19:00:207:00 pm | Malone University Kingfield |
| 2025-06-14 19:00:44June 14, 2025 | Ambassadors FC Ohio vs Buffalo Stallions | 19:00:447:00 pm | Malone University Kingfield |
| 2025-06-18 19:00:58June 18, 2025 | Ambassadors Ohio U23 vs FC Challenger | 19:00:587:00 pm | Malone University Kingfield |
| 2025-06-21 19:00:17June 21, 2025 | Ambassadors FC Ohio @ Erie Commodores FC | 19:00:177:00 pm | Mercyhurst University |
| 2025-06-25 19:00:07June 25, 2025 | Ambassadors Ohio U23 vs Canton Force | 19:00:077:00 pm | Malone University Kingfield |
| 2025-06-28 12:00:35June 28, 2025 | Ambassadors Ohio U23 vs Croatia Cleveland | 12:00:3512:00 pm | Malone University Kingfield |
| 2025-06-28 16:30:40June 28, 2025 | Ambassadors FC Ohio vs RNY FC | 16:30:404:30 pm | Malone University Kingfield |
| 2025-07-02 19:00:24July 2, 2025 | Ambassadors FC Ohio vs Erie Commodores FC | 19:00:247:00 pm | Malone University Kingfield |
| 2025-07-05 19:00:46July 5, 2025 | Ambassadors FC Ohio @ Buffalo Stallions | 19:00:467:00 pm | North Tonawanda High School |
| 2025-07-09 19:30:14July 9, 2025 | Ambassadors Ohio U23 @ Elite FC | 19:30:147:30 pm | Marysville High School Impact Stadium |
| 2025-07-12 18:00:05July 12, 2025 | Ambassadors Ohio U23 @ Erie FC | 18:00:056:00 pm | McDowell High School |
vs 
Columbus Force
