| 2025-05-14 19:30:31May 14, 2025 | Ambassadors Ohio U23 @ Croatia Cleveland | 1 - 3 | Mentor High School |
| 2025-05-17 17:00:07May 17, 2025 | Ambassadors FC Ohio @ Syracuse FC | 4 - 0 | Camden High School |
| 2025-05-24 13:00:02May 24, 2025 | Ambassadors Ohio U23 vs PFC Extreme | 3 - 0 | Malone University Kingfield |
| 2025-05-24 19:00:54May 24, 2025 | Ambassadors FC Ohio vs Flower City Union | 1 - 2 | Malone University Kingfield |
| 2025-05-28 19:00:40May 28, 2025 | Ambassadors FC Ohio vs Cleveland SC | 1 - 1 | Malone University Kingfield |
| 2025-05-31 19:00:08May 31, 2025 | Ambassadors FC Ohio @ Niagara 1812 | 1 - 0 | Niagara University |
| 2025-06-01 13:00:33June 1, 2025 | Ambassadors Ohio U23 vs FC Pittsburgh | 3 - 0 | Malone University Kingfield |
| 2025-06-04 19:00:35June 4, 2025 | Ambassadors FC Ohio @ Cleveland SC | 4 - 0 | Finnie Stadium |
| 2025-06-07 13:00:01June 7, 2025 | Ambassadors Ohio U23 vs Ohio Premier | 0 - 2 | Malone University Kingfield |
| 2025-06-11 19:00:20June 11, 2025 | Ambassadors Ohio U23 vs WV Highlanders | 2 - 2 | Malone University Kingfield |
| 2025-06-14 19:00:44June 14, 2025 | Ambassadors FC Ohio vs Buffalo Stallions | 5 - 0 | Malone University Kingfield |
| 2025-06-18 19:00:58June 18, 2025 | Ambassadors Ohio U23 vs FC Challenger | 2 - 1 | Malone University Kingfield |
| 2025-06-21 18:00:17June 21, 2025 | Ambassadors FC Ohio @ Erie Commodores FC | 1 - 3 | Mercyhurst University |
| 2025-06-25 19:00:07June 25, 2025 | Ambassadors Ohio U23 vs Canton Force | 5 - 0 | Malone University Kingfield |
| 2025-06-28 12:00:35June 28, 2025 | Ambassadors Ohio U23 vs Croatia Cleveland | 5 - 4 | Malone University Kingfield |
| 2025-06-28 16:30:40June 28, 2025 | Ambassadors FC Ohio vs RNY FC | 3 - 1 | Malone University Kingfield |
| 2025-07-02 19:00:24July 2, 2025 | Ambassadors FC Ohio vs Erie Commodores FC | 4 - 1 | Malone University Kingfield |
| 2025-07-05 19:00:46July 5, 2025 | Ambassadors FC Ohio @ Buffalo Stallions | 4 - 3 | North Tonawanda High School |
| 2025-07-09 19:00:08July 9, 2025 | Ambassadors FC Ohio vs. Rochester NY FC NPSL Conference Semifinal | 2 - 1 | Malone University Kingfield |
| 2025-07-09 19:00:14July 9, 2025 | Ambassadors Ohio U23 @ Elite FC | 5 - 2 | Marysville High School Impact Stadium |
| 2025-07-12 17:00:25July 12, 2025 | Ambassadors FC Ohio @ Flower City Union NPSL Conference Final | 0 - 3 | Rochester Community Sports Complex |
| 2025-07-13 14:00:05July 13, 2025 | Ambassadors Ohio U23 @ Erie FC | 4 - 0 | McDowell High School |
vs 
Ohio Premier
WV Highlanders
Croatia Cleveland
Elite FC
Spire FC
Erie Commodores FC
Canton Force
