| 2025-05-14 19:30:31May 14, 2025 | Ambassadors Ohio U23 @ Croatia Cleveland | 1 - 3 | Mentor High School |
| 2025-05-17 17:00:07May 17, 2025 | Ambassadors FC Ohio @ Syracuse FC | 4 - 0 | Camden High School |
| 2025-05-24 13:00:02May 24, 2025 | Ambassadors Ohio U23 vs PFC Extreme | 3 - 0 | Malone University Kingfield |
| 2025-05-24 19:00:54May 24, 2025 | Ambassadors FC Ohio vs Flower City Union | 1 - 2 | Malone University Kingfield |
| 2025-05-28 19:00:40May 28, 2025 | Ambassadors FC Ohio vs Cleveland SC | 1 - 1 | Malone University Kingfield |
| 2025-05-31 19:00:08May 31, 2025 | Ambassadors FC Ohio @ Niagara 1812 | 1 - 0 | Niagara University |
| 2025-06-01 13:00:33June 1, 2025 | Ambassadors Ohio U23 vs FC Pittsburgh | 3 - 0 | Malone University Kingfield |
| 2025-06-04 19:00:35June 4, 2025 | Ambassadors FC Ohio @ Cleveland SC | 4 - 0 | Finnie Stadium |
| 2025-06-07 13:00:01June 7, 2025 | Ambassadors Ohio U23 vs Ohio Premier | 0 - 2 | Malone University Kingfield |
| 2025-06-11 19:00:20June 11, 2025 | Ambassadors Ohio U23 vs WV Highlanders | 2 - 2 | Malone University Kingfield |
| 2025-06-14 19:00:44June 14, 2025 | Ambassadors FC Ohio vs Buffalo Stallions | 5 - 0 | Malone University Kingfield |
| 2025-06-18 19:00:58June 18, 2025 | Ambassadors Ohio U23 vs FC Challenger | 2 - 1 | Malone University Kingfield |
| 2025-06-21 18:00:17June 21, 2025 | Ambassadors FC Ohio @ Erie Commodores FC | 1 - 3 | Mercyhurst University |
| 2025-06-25 19:00:07June 25, 2025 | Ambassadors Ohio U23 vs Canton Force | 5 - 0 | Malone University Kingfield |
| 2025-06-28 12:00:35June 28, 2025 | Ambassadors Ohio U23 vs Croatia Cleveland | 5 - 4 | Malone University Kingfield |
| 2025-06-28 16:30:40June 28, 2025 | Ambassadors FC Ohio vs RNY FC | 3 - 1 | Malone University Kingfield |
| 2025-07-02 19:00:24July 2, 2025 | Ambassadors FC Ohio vs Erie Commodores FC | 4 - 1 | Malone University Kingfield |
| 2025-07-05 19:00:46July 5, 2025 | Ambassadors FC Ohio @ Buffalo Stallions | 4 - 3 | North Tonawanda High School |
| 2025-07-09 19:00:08July 9, 2025 | Ambassadors FC Ohio vs. Rochester NY FC NPSL Conference Semifinal | 2 - 1 | Malone University Kingfield |
| 2025-07-09 19:00:14July 9, 2025 | Ambassadors Ohio U23 @ Elite FC | 5 - 2 | Marysville High School Impact Stadium |
| 2025-07-12 17:00:25July 12, 2025 | Ambassadors FC Ohio @ Flower City Union NPSL Conference Final | 0 - 3 | Rochester Community Sports Complex |
| 2025-07-13 14:00:05July 13, 2025 | Ambassadors Ohio U23 @ Erie FC | 4 - 0 | McDowell High School |
Ambassadors Ohio U23
Ohio Premier
WV Highlanders
Croatia Cleveland
Flower City Union
Spire FC
Erie Commodores FC
Delaware Rising FC
Ohio City SC
Elite FC
Inter Columbus FC
Cleveland SC
Sporting Columbus
Canton Force
FC Challenger
Niagara 1812
RNY FC
FC Pittsburgh
Greater Columbus FC
PFC Extreme
Columbus Force
Syracuse FC
Villareal Erie Academy
Ashland United FC
Buffalo Stallions
Liederkranz
Erie FC
Stark County United
